| 質粒名稱: | pCDNA3 Flag MKK7B2Jnk1a1 |
|---|---|
| 出品公司: | 愛迪基因 |
| 目錄編號: | 19726 |
| 存儲實驗室: | Roger Davis |
|
載體骨架基本信息 |
|
| 載體名稱: | pcDNA3 |
| 載體出品公司: | invitrogen |
| 載體抗性: | 氨芐青霉素 |
| 空載體大小: | 5446 bp |
| 完整質粒大小: | -- |
| 載體修飾: | -- |
| 載體類型: | 哺乳細胞表達 |
| 篩選標記: | -- |
| 高拷貝/低拷貝: | 高拷貝 |
| 生長條件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名稱: | MKK7B2Jnk1a1,MKK7,JNK1{alpha}1 |
| 物種來源: | H. sapiens (human), M. musculus (mouse) |
| 插入大小: | 2546 bp |
| 基因突變: | -- |
| GenBank編號 | -- |
| 啟動子 | -- |
| 標簽/融合蛋白: | Flag（插入件上的N端子） |
| 克隆方法: | 限制性內切酶 |
| 5' 克隆位點: | Hind III(未破壞) |
| 3' 克隆位點: | Hind III(未破壞) |
| 5' 測序引物 | T7 |
| 3' 測序引物 | SP6 |
|
文獻引用方法 |
|
| 材料和方法部分: | pCDNA3 Flag MKK7B2Jnk1a1 was a gift from Roger Davis (Addgene plasmid # 19726 ; //n2t.net/addgene:19726 ; RRID:Addgene_19726) |
| 參考文獻部分: | The Bax subfamily of Bcl2-related proteins is essential for apoptotic signal transduction by c-Jun NH(2)-terminal kinase. Lei K, Nimnual A, Zong WX, Kennedy NJ, Flavell RA, Thompson CB, Bar-Sagi D, Davis RJ. Mol Cell Biol. 2002 Jul . 22(13):4929-42. 10.1128/MCB.22.13.4929-4942.2002 PubMed 12052897 |
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pCDNA3 Flag MKK7B2Jnk1a1載體質粒基本信息
pCDNA3 Flag MKK7B2Jnk1a1質粒圖譜載體圖譜和pCDNA3 Flag MKK7B2Jnk1a1載體序列質粒序列多克隆位點信息
pCDNA3 Flag MKK7B2Jnk1a1質粒載體簡介
pCDNA3 Flag MKK7B2Jnk1a1質粒序列載體序列
LOCUS pCDNA3 Flag MKK7B2Jnk1a1 8052 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCDNA3 Flag MKK7B2Jnk1a1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8052)
AUTHORS Lei K, Nimnual A, Zong WX, Kennedy NJ, Flavell RA, Thompson CB,
Bar-Sagi D, Davis RJ
TITLE The Bax subfamily of Bcl2-related proteins is essential for
apoptotic signal transduction by c-Jun NH(2)-terminal kinase.
JOURNAL Mol Cell Biol. 2002 Jul . 22(13):4929-42.
PUBMED 12052897
REFERENCE 2 (bases 1 to 8052)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..8052
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 128..507
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 508..711
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 662..682
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 756..775
/label=T7
/note="T7 promoter, forward primer"
promoter 756..774
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 802..825
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS complement(952..963)
/codon_start=1
/product="WELQut protease recognition and cleavage site"
/label=WELQut site
/translation="WELQ"
promoter complement(3498..3516)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(3499..3516)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(3536..3553)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3542..3766
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3812..4240
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3899..3918)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4109..4130
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4249..4269)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4254..4583
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4434..4569
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4496..4515
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4650..5444
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4704..4723)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5314..5333
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 5618..5739
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5655..5674)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5709..5728
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5788..5804)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5788..5804)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5801..5823)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5812..5828
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5836..5866)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5881..5902
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6019..6036)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6190..6778)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6270..6289)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6949..7809)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7572..7591
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7810..7914)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7989..8008)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
61 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
121 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
181 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
241 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
301 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
361 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
421 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
481 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
541 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
601 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
661 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
721 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
781 caagcttcgg cagccaacat ggactataag gacgatgatg acaaaatggc ggcgtcctcc
841 ctggagcaga agctgtcccg cctggaagcc aagctgaagc aggagaaccg tgaggcccgc
901 aggaggatcg acctcaactt ggatatcagc ccacagcggc ccaggcccac cctgcaactc
961 ccactggcca acgatggggg cagccgctca ccatcctcag agagctcccc acagcaccct
1021 acacccccca cccggccccg ccacatgctg gggctcccat caaccttgtt cacaccgcgc
1081 agtatggaga gcatcgagat tgaccagaag ctgcaggaga tcatgaagca gacagggtac
1141 ctgactatcg ggggccagcg ttatcaggca gaaatcaatg acttggagaa cttgggtgag
1201 atgggcagtg gtacctgtgg tcaggtgtgg aagatgcggt tccggaagac aggccacatc
1261 attgctgtta agcaaatgcg gcgctctggg aacaaggaag agaataagcg cattttgatg
1321 gacctggatg tagtactcaa gagccatgac tgcccttaca tcgttcagtg ctttggcacc
1381 ttcatcacca acacagacgt ctttattgcc atggagctca tgggcacatg tgcagagaag
1441 ctgaagaaac gaatgcaggg ccccattcca gagcgaatcc tgggcaagat gactgtggcg
1501 attgtgaaag cactgtacta tctgaaggag aagcatggcg tcatccatcg cgatgtcaaa
1561 ccctccaaca tcctgctaga tgagcggggc cagatcaagc tctgtgactt tggcatcagt
1621 ggccgccttg ttgactccaa agccaaaaca cggagtgctg gctgtgctgc ctatatggct
1681 cccgagcgca tcgaccctcc agatcccacc aagcctgact atgacatccg agctgatgtg
1741 tggagcctgg gcatctcact ggtggagctg gcaacaggac agttccccta taagaactgc
1801 aagacggact ttgaggtcct caccaaagtc ctacaggaag agcccccact cctgcctggt
1861 cacatgggct tctcagggga cttccagtca tttgtcaaag actgccttac taaagatcac
1921 aggaagagac caaagtataa taagctactt gaacacagct tcatcaagca ctatgagata
1981 ctcgaggtgg atgtcgcgtc ctggtttaag gatgtcatgg cgaagaccga gtccccaagg
2041 actagtggag tcctgagtca gcaccatctg cccttcttca gtgggagtct ggaggagtct
2101 cccacttccc caccttctcc caagtccttc cctctgtcac cagccatccc tcacgaaatt
2161 ctgagccgtc gtccgagtta ccgtaaagga tccatgagca gaagcaagcg tgacaacaat
2221 ttttatagtg tagagattgg agattctaca ttcacagtcc tgaaacgata tcagaattta
2281 aaacctatag gctcaggagc tcaaggaata gtatgcgcag cttatgatgc cattcttgaa
2341 agaaatgttg caatcaagaa gctaagccga ccatttcaga atcagactca tgccaagcgg
2401 gcctacagag agctagttct tatgaaatgt gttaatcaca aaaatataat tggccttttg
2461 aatgttttca caccacagaa atccctagaa gaatttcaag atgtttacat agtcatggag
2521 ctcatggatg caaatctttg ccaagtgatt cagatggagc tagatcatga aagaatgtcc
2581 taccttctct atcagatgct gtgtggaatc aagcaccttc attctgctgg aattattcat
2641 cgggacttaa agcccagtaa tatagtagta aaatctgatt gcactttgaa gattcttgac
2701 ttcggtctgg ccaggactgc aggaacgagt tttatgatga cgccttatgt agtgactcgc
2761 tactacagag cacccgaggt catccttggc atgggctaca aggaaaacgt ggatttatgg
2821 tctgtggggt gcattatggg agaaatggtt tgccacaaaa tcctctttcc aggaagggac
2881 tatattgatc agtggaataa agttattgaa cagcttggaa caccatgtcc tgaattcatg
2941 aagaaactgc aaccaacagt aaggacttac gttgaaaaca gacctaaata tgctggatat
3001 agctttgaga aactcttccc tgatgtcctt ttcccagctg actcagaaca caacaaactt
3061 aaagccagtc aggcaaggga tttgttatcc aaaatgctgg taatagatgc atctaaaagg
3121 atctctgtag atgaagctct ccaacacccg tacatcaatg tctggtatga tccttctgaa
3181 gcagaagctc caccaccaaa gatccctgac aagcagttag atgaaaggga acacacaata
3241 gaagagtgga aagaattgat atataaggaa gttatggact tggaggagag aaccaagaat
3301 ggagttatac gggggcagcc ctctccttta gcacaggtgc agcagtgact cgagctcaag
3361 cttcaggcca ccagtgttgc cctcttccct ttttaaaaca aaataccctt gtttgtaaat
3421 ccttagacgc ttgagaataa aacccttccc ttttcttccg aaaaaaaaaa aaaaaaaact
3481 cgaggggggg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
3541 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
3601 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
3661 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
3721 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
3781 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
3841 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
3901 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
3961 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
4021 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
4081 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
4141 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
4201 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
4261 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
4321 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
4381 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
4441 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
4501 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
4561 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
4621 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
4681 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
4741 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
4801 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
4861 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
4921 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
4981 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
5041 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
5101 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
5161 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
5221 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
5281 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
5341 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
5401 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
5461 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
5521 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
5581 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
5641 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
5701 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
5761 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
5821 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
5881 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
5941 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
6001 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
6061 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
6121 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
6181 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
6241 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
6301 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
6361 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
6421 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
6481 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
6541 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
6601 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
6661 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
6721 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
6781 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
6841 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
6901 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
6961 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
7021 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
7081 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
7141 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
7201 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
7261 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
7321 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
7381 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
7441 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
7501 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
7561 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
7621 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
7681 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
7741 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
7801 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
7861 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
7921 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
7981 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
8041 gtgttggagg tc
//
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